Fragmentary remains of Devonian fishes are described from the Knobby Sandstone, a thick non-marine clastic formation in the eastern Canning Basin, which is a potential petroleum reservoir in subsurface fault-bounded sequences overlain by marine carbonates. The fauna includes a new species of the antiarch Bothriolepis, a few dermal plates of an asterolepidoid antiarch, various coarsely ornamented placoderm fragments probably belonging to a much larger antiarch, and some isolated rhipidistian teeth and scales. The fragmentary preservation suggests a high-energy depositional environment. Associated are plant remains, including Leptophloeum australe (MCoy). All fossils are preserved as moulds in a coarse sandstone. The fauna is probably Late Devonian (Famennian) in age, but better material is required for detailed correlation with other Late Devonian vertebrate assemblages in the Canning Basin and elsewhere.